UAP1 Recombinant Mouse Monoclonal Antibody [4H1-R]
cat.: HA601660
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 4H1-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human UAP1 aa 33-82 / 522. |
| Positive control: | SK-Br-3 cell lysate, Jurkat cell lysate, NCI-H1299 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q16222 Human |
| Alternative names: | AGX 1 AGX AGX-1 AGX-2 AGX1 Antigen X AntigenX SPAG 2 SPAG2 Sperm associated antigen 2 Sperm-associated antigen 2 UAP 1 uap1 UAP1_HUMAN UDP N acetylhexosamine pyrophosphorylase UDP N acteylglucosamine pyrophosphorylase 1 UDP-N-acetylglucosamine pyrophosphorylase |
Images
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Fig1:
Western blot analysis of UAP1 on different lysates with Mouse anti-UAP1 antibody (HA601660) at 1/4,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lane 3: NCI-H1299 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 57/59 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601660) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.