Parkin Recombinant Antibody [PSH08-11] - Rat IgG1 (Chimeric)
cat.: HA601664
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clone number: | PSH08-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Immunogen: | Recombinant protein within human Parkin aa 1-200. |
| Positive control: | SH-SY5Y (Human neuroblastoma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate. |
| Subcellular location: | Mitochondrion, mitochondrion outer membrane, endoplasmic reticulum, cytosol, Nucleus, neuron projection, postsynaptic density, presynapse. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:50 1:500 |
| Uniprot #: | SwissProt: O60260 Human | Q9WVS6 Mouse | Q9JK66 Rat |
| Alternative names: | AR JP E3 ubiquitin ligase E3 ubiquitin protein ligase parkin E3 ubiquitin-protein ligase parkin FRA6E LPRS 2 LPRS2 PARK 2 Park2 Parkin 2 Parkinson disease (autosomal recessive juvenile) 2 Parkinson disease (autosomal recessive, juvenile) 2, parkin Parkinson disease protein 2 Parkinson juvenile disease protein 2 Parkinson protein 2 E3 ubiquitin protein ligase Parkinson protein 2, E3 ubiquitin protein ligase (parkin) PDJ PRKN 2 PRKN PRKN2 PRKN2_HUMAN Ubiquitin E3 ligase PRKN |
Images
|
Fig1:
Western blot analysis of Parkin on different lysates with Rat anti-Parkin antibody (HA601664) at 1/5,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: COS-1 (African green monkey kidney fibroblast) cell lysate Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Pig brain tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601664, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 52 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: Neuro-2a (Mouse brain neuroblastoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601664, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Rat Sample: PC-12 (Rat pheochromocytoma cell (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601664, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601664, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601664, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.