DUSP6 Recombinant Antibody [SR39-09] - Mouse IgG1 (Chimeric)
cat.: HA601675
| Product Type: | Recombinant Chimeric, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clone number: | SR39-09 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Immunogen: | Recombinant protein within Human DUSP6 aa 325-381 / 381. |
| Positive control: | Hep G2 (Human hepatocellular carcinoma cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C6 (Rat glioma cell) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: Q16828 Human | Q9DBB1 Mouse | Q64346 Rat |
| Alternative names: | Dual specificity phosphatase 6 Dual specificity phosphatase 6 isoform a Dual specificity protein phosphatase 6 Dual specificity protein phosphatase PYST1 DUS6_HUMAN DUSP 6 DUSP 6a Dusp6 DUSP6a HH19 MAP kinase phosphatase 3 Mitogen activated protein kinase phosphatase 3 Mitogen-activated protein kinase phosphatase 3 MKP 3 MKP-3 MKP3 PYST 1 PYST1 Serine/threonine specific protein phosphatase |
Images
|
Fig1:
Western blot analysis of DUSP6 on different lysates with Mouse anti-DUSP6 antibody (HA601675) at 1/5,000 dilution.
Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 3: C6 (Rat glioma cell) cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601675, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42 kDa Observed band size: 42 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601675, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601675, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.