CD23 Recombinant Antibody [PD00-03] - Mouse IgG1 (Chimeric)
cat.: HA601682
| Product Type: | Recombinant Chimeric, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue |
| Clone number: | PD00-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Immunogen: | Recombinant protein within human CD23 aa 48 – 321 (Extracellular). |
| Positive control: | Human Tonsil, Human Lymph node. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P06734 Human |
| Alternative names: | Blast 2 BLAST-2 Blast2 C type lectin domain family 4 member J C-type lectin domain family 4 C-type lectin domain family 4 member J C-type lectin domain family 4, member J CD 23 CD 23A CD23 CD23 antigen CD23A CLEC 4J CLEC4J Fc epsilon receptor II Fc epsilon RII Fc fragment of IgE Fc fragment of IgE low affinity II receptor for Fc fragment of IgE receptor II Fc fragment of IgE, low affinity II, receptor for (CD23) Fc of IgE Fc of IgE, low affinity II, receptor for (CD23) Fc receptor IgE low affinity II alpha polypeptide Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a Fc-epsilon-RII FCE 2 FCE2 FCER 2 Fcer2 FCER2_HUMAN FCER2A FceRII IgE binding factor IgE receptor lymphocyte IgE-binding factor IGEBF Immunoglobulin E binding factor Immunoglobulin E receptor Immunoglobulin E receptor, low affinity II Immunoglobulin E-binding factor Immunoglobulin epsilon chain LEUKOCYTE ANTIGEN CD23 Low Af...... |
Images
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Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue:Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601682, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lymph node Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601682, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601682, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.