HER2 / ErbB2 Recombinant Antibody [PD00-53] - Rat IgG1 (Chimeric)
cat.: HA601683
| Product Type: | Recombinant Chimeric, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clone number: | PD00-53 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 138 kDa |
| Immunogen: | Recombinant protein within Human ErbB2/ HER2 aa 500-650. |
| Positive control: | SK-Br-3 (Human breast cancer cell) cell lysates, Human Breast cancer. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:500 |
| Uniprot #: | SwissProt: P04626 Human |
| Alternative names: | erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog C erb B2/neu protein CD340 CD340 antigen Cerb B2/neu protein CerbB2 Erb b2 receptor tyrosine kinase 2 ErbB-2 proto-oncogene ERBB2 ERBB2_HUMAN HER 2 HER 2/NEU HER2 Herstatin Human epidermal growth factor receptor 2 Metastatic lymph node gene 19 protein MLN 19 MLN19 NEU NEU proto oncogene Neuro/glioblastoma derived oncogene homolog Neuroblastoma/glioblastoma derived oncogene homolog NGL p185erbB2 Proto-oncogene c-ErbB-2 Proto-oncogene Neu Receptor tyrosine-protein kinase erbB-2 TKR1 Tyrosine kinase type cell surface receptor HER2 Tyrosine kinase-type cell surface receptor HER2 V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 V erb b2 avian erythroblastic leukemia viral oncoprotein 2 V erb b2 erythrobl...... |
Images
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Fig1:
Western blot analysis of HER2 / ErbB2 on SK-Br-3 (Human breast cancer cell) cell lysate with Rat anti-HER2 / ErbB2 antibody (HA601683) at 1/5,000 dilution.
Lysates/proteins at 15 µg/Lane. Exposure time: 24 seconds ; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601683, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 135 kDa Observed band size: 250 kDa |
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Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601683, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast cancer Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA601683, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.