6X His tag (C-terminal) Recombinant Mouse Monoclonal Antibody [8-F0-R]
cat.: HA610015
| Product Type: | Recombinant Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | 8-F0-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG2a |
| Immunogen: | C-terminal His-tagged recombinant protein, N-terminal His-tagged recombinant protein. |
| Positive control: | C-terminal His-tag fusion protein lysates. |
| Recommended Dilutions:
WB IP |
1:5,000-1:20,000 Use at an assay dependent concentration. |
| Alternative names: | 6 His epitope tag Hexa His tag HHHHHH epitope tag HHHHHH tag His tag Polyhistidine Tag |
Images
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Fig1:
Western blot analysis of 6X His tag (C-terminal) on C-terminal His-tag fusion protein lysates with Mouse anti-6X His tag (C-terminal) antibody (HA610015) at different dilutions. Lysates/proteins at 50 ng/Lane. Observed band size: 30 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610015) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
His-tag was immunoprecipitated in 2µg C-terminal His-tag fusion protein lysate with HA610015. Western blot was performed from the immunoprecipitate using HA610015 at 1/2,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 60 mins at room temperature. Lane 1: C-terminal His-tag fusion protein lysate (input). Lane 2: HA610015 IP in C-terminal His-tag fusion protein lysate. Lane 3: Mouse IgG instead of HA610015 IP in C-terminal His-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.