FAM38A / PIEZO1 Recombinant Mouse Monoclonal Antibody [2-10-R-A]
cat.: HA610024
| Product Type: | Recombinant Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 2-10-R-A |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 287 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human PIEZO1 aa 1,780-1,980 / 2,521. |
| Positive control: | Recombinant protein lysates, rat brain tissue, mouse hippocampus tissue, mouse brain tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:200-1:1,000 1:200 |
| Uniprot #: | SwissProt: Q92508 Human | E2JF22 Mouse | Q0KL00 Rat |
| Alternative names: | DHS Fam38a Family with sequence similarity 38 member A KIAA0233 Membrane protein induced by beta-amyloid treatment Mib PIEZ1_HUMAN Piezo-type mechanosensitive ion channel component 1 PIEZO1 Protein FAM38A Protein FAM38B Protein PIEZO1 |
Images
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Fig1:
Western blot analysis of Cytokeratin 7 on different lysates with Mouse anti-Cytokeratin 7 antibody (HA601065) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: SK-OV-3 (Human ovarian cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601065, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610024) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610024) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610024) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of A431 cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (ET1602-4, Red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.