MCM7 Recombinant Mouse Monoclonal Antibody [15E1-R]
cat.: HA610161
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 15E1-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MCM7 aa 516-719 / 719. |
| Positive control: | HL-60 cell lysate, HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, SW480 cell lysate, LNCaP cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P33993 Human | Q61881 Mouse Entrez Gene: 288532 Rat |
| Alternative names: | CDABP0042 CDC 47 CDC47 CDC47 homolog Cdc47, S. cerevisiae, homolog of DNA replication licensing factor MCM7 Homolog of S. cerevisiae Cdc47 MCM 2 MCM 7 MCM2 MCM2, formerly Mcm7 MCM7 minichromosome maintenance deficient 7 MCM7_HUMAN Minichromosome Maintainence 7 Minichromosome maintainence, S. cerevisiae, homolog of Minichromosome maintenance complex component 7 Minichromosome maintenance deficient 7 Minichromosome maintenance protein 7 P1.1 MCM3 P1.1-MCM3 P1CDC47 P85MCM PNAS 146 PNAS146 PPP1R104 Protein phosphatase 1, regulatory subunit 104 |
Images
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Fig1:
Western blot analysis of MCM7 on different lysates with Mouse anti-MCM7 antibody (HA610161) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: SW480 cell lysate Lane 6: LNCaP cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.