KIAA0100 Recombinant Mouse Monoclonal Antibody [A0-F3-R]
cat.: HA610189
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A0-F3-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 254 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human KIAA0100 aa 1,947-2,235 / 2,235. |
| Positive control: | MDA-MB-231 cell lysate, SK-Br-3 cell lysate, MCF7 cell lysate, HeLa cell lysate, A431 cell lysate, PC-3M cell lysate, LNCaP cell lysate, HeLa, human breast cancer tissue, human kidney tissue, human pancreas tissue. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Mitochondrion membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:100 1:2,000 |
| Uniprot #: | SwissProt: Q14667 Human |
| Alternative names: | Antigen MLAA-22 BCOX BCOX1 Breast cancer-overexpressed gene 1 protein CT101 DKFZp686M0843 K0100_HUMAN KIAA0100 MGC111488 MGC134981 UPF0378 protein KIAA0100 |
Images
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Fig1:
Western blot analysis of KIAA0100 on different lysates with Mouse anti-KIAA0100 antibody (HA610189) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: A431 cell lysate Lane 6: PC-3M cell lysate Lane 7: LNCaP cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 254 kDa Observed band size: 55 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610189) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling KIAA0100 with Mouse anti-KIAA0100 antibody (HA610189) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-KIAA0100 antibody (HA610189) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-KIAA0100 antibody (HA610189) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610189) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-KIAA0100 antibody (HA610189) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610189) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-KIAA0100 antibody (HA610189) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610189) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.