Recombinant Mouse IgG1 protein [PSH04-91] - Isotype Control - BSA and Azide free
cat.: HA610204
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Applications: | WB, FC, IP, ChIP |
| Clonality: | Monoclonal |
| Clone number: | PSH04-91 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Small molecule. |
Images
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Fig1:
This data was developed using HA610204, the same antibody clone in a different buffer formulation. PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA601172 IP in HeLa cell lysate Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 59 seconds; ECL: K1801 |
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Fig2:
This data was developed using HA610204, the same antibody clone in a different buffer formulation. PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA601172 IP in NIH/3T3 cell lysate Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds; ECL: K1802 |
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Fig3:
This data was developed using HA610204, the same antibody clone in a different buffer formulation. PCNA was immunoprecipitated from 0.2 mg PC-12 cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PC-12 cell lysate (input) Lane 2: HA601172 IP in PC-12 cell lysate Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in PC-12 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL: K1801 |
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Fig4:
This data was developed using HA610204, the same antibody clone in a different buffer formulation. Flow cytometric analysis of HeLa cells labeling eIF-6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601293, 1μg/mL) (green) compared with Recombinant Mouse IgG1 (HA610204, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
This data was developed using HA610204, the same antibody clone in a different buffer formulation. Flow cytometric analysis of C6 cells labeling eIF-6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601293, 1μg/mL) (green) compared with Recombinant Mouse IgG1 (HA610204, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
This data was developed using HA610204, the same antibody clone in a different buffer formulation. Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H3 (acetyl K27) (HA600047) or Recombinant Mouse IgG1 (HA610204) according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.