Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29]
cat.: HA610215
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH08-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Calretinin aa 1-200. |
| Positive control: | Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue IP |
1:2,000 1:200-1:1,000 1:500 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: Q08331 Mouse | P47728 Rat |
| Alternative names: | 29 kDa calbindin CAB 29 CAB29 CAL 2 CAL2 CALB 2 CALB2 CALB2_HUMAN Calbindin 2 29kDa Calbindin 2 Calbindin D29K Calbindin2 Calretinin CR |
Images
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Fig1:
Western blot analysis of Calretinin on different lysates with Mouse anti-Calretinin antibody (HA610215) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse colon tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 32 kDa Observed band size: 29 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610215) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig3:
Application: IHC-Fr Species: Mouse Site: Retina Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA610215) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610215) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA610215) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610215) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Calretinin antibody (HA610215) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig8:
Calretinin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA610215 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA610215 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA610215 IP in mouse brain tissue lysate Lane 3: Mouse IgG instead of HA610215 in mouse brain tissue lysate Blocking/Dilution buffer: Primary Antibody Dilution Exposure time: 40 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.