c-Fos Recombinant Antibody [PSH05-77] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610219
| Product Type: | Recombinant Chimeric Antibody IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, IF-Tissue, IF-Cell, WB |
| Clone number: | PSH05-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Protein c-Fos aa 1-380. |
| Positive control: | Mouse brain tissue, rat brain tissue. |
| Subcellular location: | Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol. |
| Recommended Dilutions:
IHC-Fr IHC-P IF-Tissue IF-Cell WB |
1:2,000-1:5,000 1:5,000 1:1,000-1:2,000 1:1,000 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P01100 Human | P01101 Mouse | P12841 Rat |
| Alternative names: | Activator protein 1 AP 1 C FOS Cellular oncogene c fos Cellular oncogene fos FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) FBJ murine osteosarcoma viral oncogene homolog FBJ murine osteosarcoma viral v fos oncogene homolog FBJ Osteosarcoma Virus FOS FOS protein FOS_HUMAN G0 G1 switch regulatory protein 7 G0/G1 switch regulatory protein 7 G0S7 Oncogene FOS p55 proto oncogene c Fos Proto oncogene protein c fos Proto-oncogene c-Fos v fos FBJ murine osteosarcoma viral oncogene homolog |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/5,000 (c-Fos, HA610219, Rat, green); 1/1,000 (Iba1, ET1705-78, Rabbit, red) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer. |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2,000 (c-Fos, HA610219, Rat, green); 1/1,000 (Iba1, ET1705-78, Rabbit, red) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA610219) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA610219) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA610219) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat hippocampus (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA610219) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunocytochemistry analysis of HeLa cells untreated / HeLa cells starved 16 hours then treated with 200nM TPA for 4 hours labeling c-Fos with Rat anti-c-Fos antibody (HA610219) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-c-Fos antibody (HA610219) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig8:
Western blot analysis of c-Fos on different lysates with Rat anti-c-Fos antibody (HA610219) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41-55 kDa Exposure time: 8 seconds; ECL: K1801; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610219) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rat IgG - HRP Secondary Antibody (HA1023) at 1/10,000 dilution was used for 1 hour at room temperature. |
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Fig9:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Cerebral cortex (restraint stress induced) Sample: Frozen section Antigen retrieval: 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: c-Fos, 1/5,000 (HA610219, rat, Green), Neurogranin, 1/500 (HA721915, rabbit, Red), overnight at 4℃. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), Goat anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.