c-Fos Recombinant Antibody [PSH05-77] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
cat.: HA610220
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, WB |
| Clone number: | PSH05-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Immunogen: | Recombinant protein within human Protein c-Fos aa 1-380. |
| Positive control: | Mouse brain tissue, rat brain tissue. |
| Subcellular location: | Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol. |
| Recommended Dilutions:
IHC-Fr WB |
1:2,000-1:5,000 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P01100 Human | P01101 Mouse | P12841 Rat |
| Alternative names: | Activator protein 1 AP 1 C FOS Cellular oncogene c fos Cellular oncogene fos FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) FBJ murine osteosarcoma viral oncogene homolog FBJ murine osteosarcoma viral v fos oncogene homolog FBJ Osteosarcoma Virus FOS FOS protein FOS_HUMAN G0 G1 switch regulatory protein 7 G0/G1 switch regulatory protein 7 G0S7 Oncogene FOS p55 proto oncogene c Fos Proto oncogene protein c fos Proto-oncogene c-Fos v fos FBJ murine osteosarcoma viral oncogene homolog |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2,000 (c-Fos, HA610220, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green) Antigen retrieval: Not required Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer. |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2,000 (c-Fos, HA610220, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green) Antigen retrieval: Not required Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer. |
|
Fig3:
Western blot analysis of c-Fos on different lysates with Guinea pig anti-c-Fos antibody (HA610220) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41-55 kDa Exposure time: 6 seconds; ECL: K1801; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610220) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/10,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Cerebral cortex (restraint stress induced) Sample: Frozen section Antigen retrieval: 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: c-Fos, 1/5,000 (HA610220, guinea pig, Green), Neurogranin, 1/500 (HA721915, rabbit, Red), overnight at 4℃. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), Goat anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.