Iba1 Recombinant Antibody [JM36-62] - Chicken IgY (Chimeric) - BSA and Azide free
cat.: HA610221
| Product Type: | Recombinant Chimeric Antibody IgY, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Pig, Cynomolgus monkey |
| Applications: | IHC-Fr, WB, IF-Cell, FC |
| Clone number: | JM36-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgY |
| Immunogen: | Synthetic peptide within N-terminal human Iba1. |
| Positive control: | Mouse cerebellum tissue, mouse spleen tissue, THP-1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, C6, THP-1. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup. |
| Recommended Dilutions:
IHC-Fr WB IF-Cell FC |
1:500 1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P55008 Human | O70200 Mouse | P55009 Rat |
| Alternative names: | AIF 1 AIF-1 Aif1 AIF1 protein AIF1_HUMAN Allograft inflammatory factor 1 Allograft inflammatory factor 1 splice variant G allograft inflammatory factor-1 splice variant Hara-1 balloon angioplasty responsive transcription BART 1 G1 G1 putative splice variant of allograft inflamatory factor 1 IBA 1 IBA1 interferon gamma responsive transcript Interferon responsive transcript 1 interferon responsive transcript factor 1 Ionized calcium binding adapter molecule 1 Ionized calcium-binding adapter molecule 1 ionized calcium-binding adapter molecule IRT 1 IRT1 Microglia response factor MRF1 Protein g1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/500 (MAG, HA721818, red) Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/500 (PSD95, ET1602-20, red) Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/1,000 (F4/80, HA721520, red) Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Rat Site: Spleen Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/1,000 (F4/80, HA721520, red) Antigen retrieval: Not required |
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Fig5:
Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody (HA610221) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610221) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (HA1146) at 1/10,000 dilution was used for 1 hour at room temperature. |
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Fig6:
Immunocytochemistry analysis of C6 cells labeling Iba1 with Chicken anti-Iba1 antibody (HA610221) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Chicken anti-Iba1 antibody (HA610221) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Chicken IgY (iFluor™ 647, HA1142) was used as the secondary antibody at 1/250 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig7:
Flow cytometric analysis of THP-1 cells labeling Iba1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA610221, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Chicken IgY Secondary antibody (HA1145) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.