QKI Recombinant Mouse Monoclonal Antibody [PSH10-21]
cat.: HA610222
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, IHC-Fr, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH10-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human QKI aa 1-341 / 341. |
| Positive control: | HeLa cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, HeLa, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P IHC-Fr IP |
1:2,000 1:100 1:200 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: Q96PU8 Human | Q9QYS9 Mouse | Q91XU1 Rat |
| Alternative names: | DKFZp586I0923 HKQ Homolog of mouse quaking QKI KH domain RNA binding protein Hqk HQK1 HqkI OTTHUMP00000017581 OTTHUMP00000017582 OTTHUMP00000017583 Protein quaking QK QK1 QK3 QKI QKI_HUMAN QKI1 Quaking homolog Quaking homolog KH domain RNA binding Quaking homolog KH domain RNA binding mouse Quaking isoform 1 Quaking protein RNA binding protein HQK |
Images
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Fig1:
Western blot analysis of QKI on different lysates with Mouse anti-QKI antibody (HA610222) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Rat cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 38 kDa Observed band size: 35-38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610222) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling QKI with Mouse anti-QKI antibody (HA610222) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-QKI antibody (HA610222) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunofluorescence analysis of frozen mouse cerebellum tissue with Mouse anti-QKI antibody (HA610222) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610222, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI antibody (HA610222) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610222) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-QKI antibody (HA610222) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610222) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
QKI was immunoprecipitated from 0.2 mg HeLa cell lysate with HA610222 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA610222 at 1/1,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA610222 IP in HeLa cell lysate Lane 3: Mouse IgG instead of HA610222 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
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