GABRA1 Recombinant Mouse Monoclonal Antibody [PSH10-46]
cat.: HA610227
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH10-46
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human GABRA1 aa 334-421.
Positive control: Mouse cerebellum tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate.
Subcellular location: Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: P62812 Mouse | P62813 Rat
Alternative names: ECA4 EIEE19 EJM EJM5 Gaba receptor alpha 1 polypeptide GABA(A) receptor GABA(A) receptor subunit alpha 1 GABA(A) receptor subunit alpha-1 GABA(A) receptor, alpha 1 GABRA 1 GABRA1 Gamma aminobutyric acid (GABA) A receptor alpha 1 Gamma aminobutyric acid A receptor alpha 1 Gamma aminobutyric acid receptor subunit alpha 1 Gamma aminobutyric acid type A receptor alpha1 subunit Gamma-aminobutyric acid receptor subunit alpha-1 GBRA1_HUMAN
Images
HA610227_1.jpg Fig1: Western blot analysis of GABRA1 on different lysates with Mouse anti-GABRA1 antibody (HA610227) at 1/2,000 dilution.

Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse lung tissue lysate (negative)
Lane 4: Rat cerebellum tissue lysate
Lane 5: Rat brain tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610227) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.