| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH10-46 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human GABRA1 aa 334-421. |
| Positive control: | Mouse cerebellum tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P62812 Mouse | P62813 Rat |
| Alternative names: | ECA4 EIEE19 EJM EJM5 Gaba receptor alpha 1 polypeptide GABA(A) receptor GABA(A) receptor subunit alpha 1 GABA(A) receptor subunit alpha-1 GABA(A) receptor, alpha 1 GABRA 1 GABRA1 Gamma aminobutyric acid (GABA) A receptor alpha 1 Gamma aminobutyric acid A receptor alpha 1 Gamma aminobutyric acid receptor subunit alpha 1 Gamma aminobutyric acid type A receptor alpha1 subunit Gamma-aminobutyric acid receptor subunit alpha-1 GBRA1_HUMAN |
|
Fig1:
Western blot analysis of GABRA1 on different lysates with Mouse anti-GABRA1 antibody (HA610227) at 1/2,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse lung tissue lysate (negative) Lane 4: Rat cerebellum tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610227) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |