SOX2 Recombinant Antibody [PO00-28] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610236
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications: IHC-Fr, IHC-P, WB, IF-Cell
Clone number: PO00-28
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 34 kDa
Immunogen: Recombinant protein within human SOX2 aa 1-317.
Positive control: Mouse E14.5 embryo lung tissue, mouse E14.5 embryo tissue, rat E14.5 embryo lung tissue, rat brain tissue, human trachea tissue, NCCIT cell lysate, F9 cell lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  IHC-Fr
  IHC-P
  WB
  IF-Cell
1:500
1:1,000
1:2,000
1:100
Uniprot #: SwissProt: P48431 Human | P48432 Mouse
Entrez Gene: 499593 Rat
Alternative names: ANOP3 cb236 Delta EF2a lcc MCOPS3 MGC148683 MGC2413 RGD1565646 Sex determining region Y box 2 SOX 2 Sox2 SOX2_HUMAN SRY (sex determining region Y) box 2 SRY box containing gene 2 SRY related HMG box 2 SRY related HMG box gene 2 SRY-box 2 Transcription factor SOX 2 Transcription factor SOX-2 ysb
Images
HA610236_1.jpg Fig1: Application: IHC-Fr

Species: Mouse

Site: E14.5 embryonic brain

Sample: Frozen section

Antibody concentration: 1/500

Antigen retrieval: Not required
HA610236_2.jpg Fig2: Application: IHC-Fr

Species: Mouse

Site: Cerebral cortex

Sample: Frozen section

Antibody concentration: 1/500

Antigen retrieval: Not required
HA610236_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo lung tissue with Rat anti-SOX2 antibody (HA610236) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610236) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA610236_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue with Rat anti-SOX2 antibody (HA610236) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610236) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA610236_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat E14.5 embryo lung tissue with Rat anti-SOX2 antibody (HA610236) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610236) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA610236_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-SOX2 antibody (HA610236) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610236) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA610236_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rat anti-SOX2 antibody (HA610236) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610236) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA610236_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rat anti-SOX2 antibody (HA610236) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610236) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA610236_9.jpg Fig9: Western blot analysis of SOX2 on different lysates with Rat anti-SOX2 antibody (HA610236) at 1/2,000 dilution.

Lane 1: NCCIT cell lysate (15 µg/Lane)
Lane 2: F9 cell lysate (15 µg/Lane)

Predicted band size: 34 kDa
Observed band size: 36 kDa

Exposure time: 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610236) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
HA610236_10.jpg Fig10: Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: F9 (Mouse teratocarcinoma cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA610236, 1/100, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.