Dopamine Transporter Recombinant Antibody [PSH10-49] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610244
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH10-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa. |
| Immunogen: | Recombinant protein. |
| Positive control: | Mouse striatum tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:500-1:2,000 1:2,000 |
| Uniprot #: | SwissProt: Q61327 Mouse | P23977 Rat |
| Alternative names: | DA transporter DAT 1 DAT DAT1 Dopamine transporter 1 Dopamine transporter PKDYS SC6A3_HUMAN SLC6A3 Sodium dependent dopamine transporter Sodium-dependent dopamine transporter Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 Solute carrier family 6 (neurotransmitter transporter), member 3 Solute carrier family 6 member 3 Variable number tandem repeat (VNTR) |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Dopamine Transporter antibody (HA610244) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610244) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Dopamine Transporter antibody (HA610244) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610244) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of Dopamine Transporter on different lysates with Rat anti-Dopamine Transporter antibody (HA610244) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610244) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.