BRN3A Recombinant Antibody [PSH11-65] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610252
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-Fr, IHC-P |
| Clone number: | PSH11-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Immunogen: | Recombinant protein within Mouse BRN3A aa 1-421. |
| Positive control: | Mouse embryo tissue, mouse embryonic eye tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:50 |
| Uniprot #: | SwissProt: P17208 Mouse |
| Alternative names: | Brain specific homeobox/POU domain protein 3A Brain-3A Brain-specific homeobox/POU domain protein 3A Brn 3.0 BRN 3A Brn-3A Brn3 BRN3A class 4 FLJ13449 Homeobox/POU domain protein RDC 1 Homeobox/POU domain protein RDC-1 Homeobox/POU domain protein RDC1 Oct T1 Oct-T1 OctT1 PO4F1_HUMAN POU class 4 homeobox 1 POU domain POU Domain Class 4 Transcription Factor 1 Pou4f1 RDC 1 RDC1 transcription factor 1 |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse embryo tissue with Rat anti-BRN3A antibody (HA610252) at 1/500 dilution. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610252, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse embryonic eye tissue with Rat anti-BRN3A antibody (HA610252) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610252) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.