Homer1 Recombinant Antibody [JE65-19] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610255
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | JE65-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Immunogen: | Recombinant protein within human Homer1 aa 101-250/354. |
| Positive control: | Mouse brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, postsynaptic density, synapse, dendritic spine. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:1,000 1:5,000 |
| Uniprot #: | SwissProt: Q9Z2Y3 Mouse | Q9Z214 Rat |
| Alternative names: | HOME1_HUMAN Homer HOMER 1A HOMER 1B HOMER 1C Homer homolog 1 (Drosophila) homer homolog 1 Homer protein homolog 1 homer scaffolding protein 1 Homer, Drosophila, homolog of, 1 Homer, neuronal immediate early gene, 1 Homer-1 Homer1 HOMER1A HOMER1B HOMER1C SYN47 Ves 1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Homer1 antibody (HA610255) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue (negative) with Rat anti-Homer1 antibody (HA610255) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Homer1 antibody (HA610255) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of Homer1 on different lysates with Rat anti-Homer1 antibody (HA610255) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (low expression) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (low expression) (20 µg/Lane) Predicted band size: 40 kDa Observed band size: 46 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610255) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. Low expression: heart, kidney and lung (PMID: 19046353). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.