alpha smooth muscle Actin Recombinant Antibody [SY02-64] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610267
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC(Intra) |
| Clone number: | SY02-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Immunogen: | Synthetic peptide within N-terminal human alpha smooth muscle Actin. |
| Positive control: | Saos-2 (Human osteosarcoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C2C12 (Mouse myoblast) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, Mouse skin tissue lysate, Rat skin tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC(Intra) |
1:5,000 1:5,000 1:50,000 1:1,000 |
| Uniprot #: | SwissProt: P62736 Human | P62737 Mouse | P62738 Rat |
| Alternative names: | alpha SMA a-SMA asma a actin AAT6 ACTA_HUMAN ACTA2 Actin alpha 2 smooth muscle aorta Actin aortic smooth muscle Actin, aortic smooth muscle ACTSA ACTVS Alpha 2 actin Alpha actin 2 Alpha cardiac actin Alpha-actin-2 Cell growth inhibiting gene 46 protein Cell growth-inhibiting gene 46 protein GIG46 Growth inhibiting gene 46 MYMY5 |
Images
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Fig1:
Western blot analysis of alpha smooth muscle Actin on different lysates with Rat anti-alpha smooth muscle Actin antibody (HA610267) at 1/5,000 dilution. Lane 1: Saos-2 (Human osteosarcoma cell) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: C2C12 (Mouse myoblast) cell lysate Lane 5: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate Lane 6: Mouse skin tissue lysate Lane 7: Rat skin tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610267, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42 kDa Observed band size: 42 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: NIH/3T3 (Mouse fibroblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610267, 1/5,000, overnight at 4℃. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Small intestine Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610267, 1/50,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
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Fig4:
Application: Flow Cytometry (Intra) Species: Human Sample: Saos-2 (Human osteosarcoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA610267 (1/1,000, Red), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 15 minutes at room temperature. |
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Fig5:
Application: Flow Cytometry (Intra) Species: Mouse Sample: NIH/3T3 (Mouse fibroblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA610267 (1/1,000, Red), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.