VGLUT1 Recombinant Antibody [PSH10-53] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610284
| Product Type: | Recombinant Chimeric, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH10-53 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Immunogen: | Recombinant protein within Mouse VGLUT1 aa 1-560. |
| Positive control: | Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate. |
| Subcellular location: | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome. |
| Uniprot #: | SwissProt: Q3TXX4 Mouse | Q62634 Rat |
| Alternative names: | BNPI Brain specific Na (+) dependent inorganic phosphate cotransporter Brain specific Na dependent inorganic phosphate cotransporter Brain-specific Na(+)-dependent inorganic phosphate cotransporter Slc17a7 Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 Solute carrier family 17 member 7 Vesicular glutamate transporter 1 VGLU1_HUMAN VGLUT 1 VGluT1 |
Images
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Fig1:
This data was developed using HA610284, the same antibody clone in a different buffer formulation. mIHC analysis of mouse brain tissue (Frozen section) with VGLUT1 (HA610284, 1/2,000) and antibody MAP2 (HA723025, 1/20,000). The immunostaining was performed with the IRISKitCmTSA Kit (900802). Blocking with 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig2:
This data was developed using HA610284, the same antibody clone in a different buffer formulation. Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig3:
This data was developed using HA610284, the same antibody clone in a different buffer formulation. Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
This data was developed using HA610284, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT1 antibody (HA610284) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610284) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
This data was developed using HA610284, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT1 antibody (HA610284) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610284) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
This data was developed using HA610284, the same antibody clone in a different buffer formulation. Western blot analysis of VGLUT1 on different lysates with Mouse anti-VGLUT1 antibody (HA610284) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse lung tissue lysate (no heat) (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610284) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.