GAD65 Recombinant Antibody [PSH06-80] - Chicken IgY (Chimeric) - BSA and Azide free
cat.: HA610307
| Product Type: | Recombinant Chimeric Antibody IgY, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | PSH06-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgY |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
IHC-Fr WB |
1:200 1:5,000 |
| Uniprot #: | SwissProt: P48320 Mouse | Q05683 Rat |
| Alternative names: | 65 kDa glutamic acid decarboxylase DCE 2 DCE2 DCE2_HUMAN GAD 2 GAD 65 GAD-2 GAD-65 Gad2 Glutamate decarboxylase 2 (pancreas) Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) Glutamate decarboxylase 2 Glutamate Decarboxylase 65 Glutamate decarboxylase 65 kDa isoform Glutamic Acid Decarboxylase 2 Glutamic Acid Decarboxylase 65 MGC161605 MGC161607 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig2:
Western blot analysis of GAD65 on different lysates with Chicken anti-GAD65 antibody (HA610307) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610307) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.