VAChT / SLC18A3 Recombinant Antibody [PSH10-50] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610311
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clone number: | PSH10-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Immunogen: | Recombinant protein within N terminal Mouse VAChT. |
| Positive control: | Mouse brain (striatum) tissue, rat brain (striatum) tissue. |
| Subcellular location: | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:200 1:500 |
| Uniprot #: | SwissProt: O35304 Mouse | Q62666 Rat |
| Alternative names: | AChR MGC12716 rVAT Slc18a3 Solute carrier family 18 (vesicular acetylcholine) member 3 Solute carrier family 18 (vesicular monoamine) member 3 Solute carrier family 18 member 3 VAChT VACHT_HUMAN Vesicular acetylcholine transporter |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain (striatum) Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (HA610311) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610311) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain (striatum) tissue with Mouse anti-VAChT / SLC18A3 antibody (HA610311) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610311) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.