NeuN Recombinant Antibody [SR45-07] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
cat.: HA610315
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Mouse, Rat
Applications: IHC-Fr, WB, IHC-P, IF-Tissue
Clone number: SR45-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 34 kDa
Immunogen: Synthetic peptide within human NeuN aa 20-60.
Positive control: Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  IHC-Fr
  WB
  IHC-P
  IF-Tissue
1:1,000
1:10,000
1:1,000
1:500
Uniprot #: SwissProt: Q8BIF2 Mouse
Unigene: 143966 Rat
Alternative names: FLJ56884 FLJ58356 Fox-1 homolog C fox1 homolog C Fox3 FOX3NeuN hexaribonucleotide binding protein 3 HRNBP3 NEUN neuronal nuclei Rbfox3 RFOX3_HUMAN RNA binding protein fox-1 homolog 3 RNA binding protein, fox 1 homolog (C. elegans) 3
Images
HA610315_1.jpg Fig1: Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Cerebral cortex
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA610315, 1/1,000, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.
HA610315_2.jpg Fig2: Western blot analysis of NeuN on different lysates with Guinea pig anti-NeuN antibody (HA610315) at 1/10,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: C2C12 cell lysate (negative)
Lane 3: Rat brain tissue lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 34 kDa
Observed band size: 45/50 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610315) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
HA610315_3.jpg Fig3: Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610315, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1021, 30 minutes at room temperature.
HA610315_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610315, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1021, 30 minutes at room temperature.
HA610315_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610315, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1021, 30 minutes at room temperature.
HA610315_6.jpg Fig6: Application: Immunofluorescence (IF-tissue)

Species: Human
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610315, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.
HA610315_7.jpg Fig7: Application: Immunofluorescence (IF-tissue)

Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610315, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.
HA610315_8.jpg Fig8: Application: Immunofluorescence (IF-tissue)

Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA610315, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.