TPH2 Recombinant Antibody [PSH04-89] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
cat.: HA610322
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, WB |
| Clone number: | PSH04-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Immunogen: | Recombinant protein with Human THP2 aa 1-490. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytosol, neuron projection. |
| Recommended Dilutions:
IHC-Fr WB |
1:200-1:500 1:5,000 |
| Uniprot #: | SwissProt: Q8CGV2 Mouse | Q8CGU9 Rat |
| Alternative names: | MGC138871 ADHD7 FLJ37295 MGC138872 Neuronal tryptophan hydroxylase NTPH TPH 2 Tph2 TPH2_HUMAN Tryptophan 5-hydroxylase 2 Tryptophan 5-monooxygenase 2 Tryptophan hydroxylase 2 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Raphe nucleus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig2:
Western blot analysis of TPH2 on different lysates with Guinea pig anti-TPH2 antibody (HA610322) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate (negative) Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610322) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.