SOX2 Recombinant Antibody [PO00-28] - Chicken IgY (Chimeric) - BSA and Azide free
cat.: HA610329
| Product Type: | Recombinant Chimeric Antibody IgY, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-Fr, WB |
| Clone number: | PO00-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgY |
| Immunogen: | Recombinant protein within human SOX2 aa 1-317. |
| Positive control: | Mouse cerebral cortex, NCCIT cell lysate, F9 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-Fr WB |
1:200 1:5,000 |
| Uniprot #: | SwissProt: P48431 Human | P48432 Mouse |
| Alternative names: | ANOP3 cb236 Delta EF2a lcc MCOPS3 MGC148683 MGC2413 RGD1565646 Sex determining region Y box 2 SOX 2 Sox2 SOX2_HUMAN SRY (sex determining region Y) box 2 SRY box containing gene 2 SRY related HMG box 2 SRY related HMG box gene 2 SRY-box 2 Transcription factor SOX 2 Transcription factor SOX-2 ysb |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
|
Fig2:
Western blot analysis of SOX2 on different lysates with Chicken anti-SOX2 antibody (HA610329) at 1/5,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610329) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Chicken IgY - HRP Secondary Antibody (HA1046) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.