Aldolase A/B/C Recombinant Antibody [PSH01-81] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610341
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH01-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Immunogen: | Synthetic peptide within Human Aldolase C aa 50-100. |
| Positive control: | Mouse brain tissue, rat brain tissue, U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | cytoskeleton, cytosol, extracellular exosome, extracellular region |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:5,000 1:5,000 |
| Uniprot #: | SwissProt: P09972 Human | P05063 Mouse | P09117 Rat |
| Alternative names: | ALDC ALDO C aldoc ALDOC_HUMAN Aldolase 3 Aldolase C Fructose bisphosphate Brain type aldolase Brain-type aldolase Fructoaldolase C Fructose 1 6 biphosphate triosephosphate lyase Fructose bisphosphate aldolase C Fructose-bisphosphate aldolase C |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Aldolase A/B/C antibody (HA610341) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610341) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Aldolase A/B/C antibody (HA610341) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610341) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of Aldolase A/B/C on different lysates with Mouse anti-Aldolase A/B/C antibody (HA610341) at 1/5,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C6 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse pancreas tissue lysate (negative) Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610341) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.