Myc tag Recombinant Mouse Monoclonal Antibody [PSH14-94]
cat.: HA610343
| Product Type: | Recombinant Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH14-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 0.1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Recommended Dilutions:
WB IF-Cell IP |
1:20,000-1:50,000 1:200 1-2μg/sample |
| Alternative names: | c-myc tag Myc Epitope Tag |
Images
|
Fig1:
Western blot analysis of Myc tag on different lysates with Mouse anti-Myc tag antibody (HA610343) at 1/20,000 dilution. Lane 1: 293T transfected with Myc-tagged ACAT2 (C-terminal) cell lysate Lane 2: 293T transfected with Myc-tagged Claudin18.2 (C-terminal) cell lysate Lane 3: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610343) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling Myc tag with Mouse anti-Myc tag antibody (HA610343) at 1/200 dilution. HeLa cells, transfected with empty control (top, negative) / Myc-tagged Claudin18.2 (C-terminal) (middle, positive) / Myc-tagged Histone H3.1 (N-terminal) (bottom, positive) expression vector, respectively, were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myc tag antibody (HA610343) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
|
Fig3:
Myc tag was immunoprecipitated from 0.2 mg 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate with HA610343 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA610343 at 1/20,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate (input) Lane 2: HA610343 IP in 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate Lane 3: Mouse IgG instead of HA610343 in 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.