Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610348
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, IF-Tissue, WB |
| Clone number: | SA0377 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Immunogen: | Synthetic peptide within Human GLUT1 aa 443-492 / 492. |
| Positive control: | Human liver tissue, mouse liver tissue, HeLa cell lysate, HT-29 cell lysate, PC-12 cell lysate, Mouse liver tissue lysate. |
| Subcellular location: | Cell membrane, Melanosome |
| Recommended Dilutions:
IHC-Fr IHC-P IF-Tissue WB |
1:500 1:2,000-1:5,000 1:500-1:1,000 1:50,000 |
| Uniprot #: | SwissProt: P11166 Human | P17809 Mouse | P11167 Rat |
| Alternative names: | Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) CSE DYT17 DYT18 DYT9 EIG12 erythrocyte/brain Erythrocyte/hepatoma glucose transporter facilitated glucose transporter member 1 Glucose transporter 1 Glucose transporter type 1 Glucose transporter type 1, erythrocyte/brain GLUT GLUT-1 GLUT1 GLUT1DS GLUTB GT1 GTG1 Gtg3 GTR1_HUMAN HepG2 glucose transporter HTLVR Human T cell leukemia virus (I and II) receptor MGC141895 MGC141896 PED RATGTG1 Receptor for HTLV 1 and HTLV 2 SLC2A1 Solute carrier family 2 (facilitated glucose transporter), member 1 Solute carrier family 2 Solute carrier family 2, facilitated glucose transporter member 1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA610348) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610348) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA610348) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610348) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of Glucose Transporter GLUT1 on different lysates with Rat anti-Glucose Transporter GLUT1 antibody (HA610348) at 1/50,000 dilution. Lane 1: HeLa cell lysate Lane 2: HT-29 cell lysate Lane 3: PC-12 cell lysate Lane 4: Mouse liver tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610348) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.