MCM2 Recombinant Antibody [PD00-90] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610350
| Product Type: | Recombinant Chimeric Antibody IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, IHC-P |
| Clone number: | PD00-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | HeLa cell lysate, 293T cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa, human tonsil tissue. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P49736 Human | P97310 Mouse Entrez Gene: 312538 Rat |
| Alternative names: | BM28 CCNL 1 CCNL1 CDC like 1 cdc19 CDCL 1 CDCL1 Cell devision cycle like 1 Cyclin like 1 D3S3194 DNA replication licensing factor MCM2 KIAA0030 MCM 2 MCM2 MCM2 minichromosome maintenance deficient 2 mitotin MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) MCM2 minichromosome maintenance deficient 2, mitotin MCM2_HUMAN MGC10606 Minichromosome maintenance complex component 2 Minichromosome maintenance deficient 2 (mitotin) Minichromosome maintenance deficient 2 mitotin Minichromosome maintenance protein 2 Minichromosome maintenance protein 2 homolog Mitotin Nuclear protein BM28 OTTHUMP00000216047 OTTHUMP00000216050 |
Images
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Fig1:
Western blot analysis of MCM2 on different lysates with Mouse anti-MCM2 antibody (HA610350) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610350) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling MCM2 with Mouse anti-MCM2 antibody (HA610350) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MCM2 antibody (HA610350) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MCM2 antibody (HA610350) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610350) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.