LC3B Recombinant Antibody [JJ090-6] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610352
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clone number: | JJ090-6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14/16 kDa |
| Immunogen: | Synthetic peptide within human LC3 B aa 1-20. |
| Positive control: | HeLa cell lysate, HeLa treated with 50μM Chloroquine for 18 hours cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HeLa cells treated with 50μM Chloroquine for 18 hours, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:200 1:2,000 |
| Uniprot #: | SwissProt: Q9GZQ8 Human | Q9CQV6 Mouse | Q62625 Rat |
| Alternative names: | ATG8F Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B LC3B LC3II MAP1 light chain 3 like protein 2 MAP1 light chain 3-like protein 2 MAP1A/1BLC3 MAP1A/MAP1B LC3 B MAP1A/MAP1B light chain 3 B MAP1ALC3 MAP1LC3B a Map1lc3b Microtubule associated protein 1 light chain 3 beta Microtubule-associated protein 1 light chain 3 beta Microtubule-associated proteins 1A/1B light chain 3B MLP3B_HUMAN |
Images
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Fig1:
Western blot analysis of LC3B on different lysates with Mouse anti-LC3B antibody (HA610352) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 14/16 kDa Observed band size: 14/16 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610352) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells untreated / treated with 50μM Chloroquine for 18 hours labeling LC3B with Mouse anti-LC3B antibody (HA610352) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LC3B antibody (HA610352) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-LC3B antibody (HA610352) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610352) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-LC3B antibody (HA610352) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610352) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.