CD20 Recombinant Antibody [PD00-02] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610360
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IHC-Fr |
| Clone number: | PD00-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Immunogen: | Synthetic peptide within Human CD20 aa 210-297 (Cytoplasmic). |
| Positive control: | Raji cell lysate, Ramos cell lysate, Daudi cell lysate, human tonsil tissue, mouse spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:5,000 1:20,000 1:500 |
| Uniprot #: | SwissProt: P11836 Human | P19437 Mouse |
| Alternative names: | APY ATOPY B lymphocyte antigen CD20 B Lymphocyte Cell Surface Antigen B1 B-lymphocyte antigen CD20 B-lymphocyte cell-surface antigen B1 B-lymphocyte surface antigen B1 B1 Bp 35 Bp35 CD 20 CD20 CD20 antigen CD20 receptor CD20_HUMAN CVID 5 CVID5 Fc epsilon receptor I beta chain Fc Fragment of IgE high affinity I receptor for beta polypeptide FCER1B High affinity immunoglobulin epsilon receptor subunit beta IgE Fc receptor subunit beta IGEL IGER IGHER LEU 16 Leu-16 LEU16 leukocyte surface antigen Leu 16 Leukocyte surface antigen Leu-16 Ly44 Membrane spanning 4 domains A1 Membrane spanning 4 domains subfamily A member 2 membrane-spanning 4-domains A1 Membrane-spanning 4-domains subfamily A member 1 MGC3969 MS4A1 MS4A2 S7 |
Images
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Fig1:
Western blot analysis of CD20 on different lysates with Mouse anti-CD20 antibody (HA610360) at 1/5,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (negative) (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610360) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD20 antibody (HA610360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD20 antibody (HA610360) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610360) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.