CD11c Recombinant Antibody [SI19-06] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610361
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Tissue, IHC-P |
| Clone number: | SI19-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 128 kDa |
| Immunogen: | Synthetic peptide within Human CD11c aa 1,114-1,163 / 1,163 (Cytoplasmic). |
| Positive control: | Human spleen tissue, human Hodgkin's lymphoma tissue, human tonsil tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IF-Tissue IHC-P |
1:500 1:800 |
| Uniprot #: | SwissProt: P20702 Human |
| Alternative names: | 95 alpha chain 95 CD 11c CD11 antigen-like family member C CD11c CD11c antigen Complement component 3 receptor 4 subunit CR4 Integrin alpha X Integrin alpha X chain Integrin alpha-X Integrin aX Integrin subunit alpha X integrin, alpha X (antigen CD11C (p150), alpha polypeptide) integrin, alpha X (complement component 3 receptor 4 subunit ITAX_HUMAN ITGAX LEU M5 alpha subunit Leu M5 Leukocyte adhesion glycoprotein p150 95 alpha chain Leukocyte adhesion glycoprotein p150 Leukocyte adhesion receptor p150 95 Leukocyte adhesion receptor p150 Leukocyte surface antigen p150 95 alpha subunit Leukocyte surface antigen p150 alpha subunit Myeloid membrane antigen alpha subunit p150 95 integrin alpha chain p150 p150/95 SLEB6 |
Images
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Fig1:
Application: IF-Tissue Species: Human Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11c antibody (HA610361) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610361) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Mouse anti-CD11c antibody (HA610361) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610361) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11c antibody (HA610361) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610361) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.