Calreticulin Recombinant Antibody [SU37-03] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610363
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, IF-Cell |
| Clone number: | SU37-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Immunogen: | Synthetic peptide within Human Calreticulin aa 40-89 / 417. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human breast carcinoma tissue, human liver tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Endoplasmic reticulum lumen, Cytoplasm, Secreted, Cell surface, Sarcoplasmic reticulum lumen, nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:10,000 1:20,000 1:1,000 |
| Uniprot #: | SwissProt: P27797 Human | P14211 Mouse | P18418 Rat |
| Alternative names: | Autoantigen RO CALR CALR protein CALR_HUMAN Calregulin Calreticulin cC1qR CRP55 CRT CRTC Endoplasmic reticulum resident protein 60 Epididymis secretory sperm binding protein Li 99n ERp60 FLJ26680 grp60 HACBP HEL S 99n RO Sicca syndrome antigen A (autoantigen Ro; calreticulin) Sicca syndrome antigen A SSA |
Images
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Fig1:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: Ice cold 100% methanol, 5 minutes at room temperature. Permeabilization: / Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610363, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
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Fig2:
Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (HA610363) at 1/10,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: COS-1 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610363) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Calreticulin antibody (HA610363) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610363) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Calreticulin antibody (HA610363) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610363) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Calreticulin antibody (HA610363) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610363) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Calreticulin antibody (HA610363) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610363) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.