Smad7 Recombinant Mouse Monoclonal Antibody [PSH21-17]
cat.: HA610387
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH21-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG1 |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, C6 cell lysate, NR8383 cell lysate, Rat liver tissue lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O15105 Human | O35253 Mouse | O88406 Rat |
| Alternative names: | Mothers against decapentaplegic homolog 7 MAD homolog 7 Mothers against DPP homolog 7 Mothers against decapentaplegic homolog 8 MAD homolog 8 Mothers against DPP homolog 8 SMAD family member 7 SMAD 7 Smad7 hSMAD7 SMAD7 MADH7 MADH8 |
Images
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Fig1:
Western blot analysis of Smad7 on different lysates with Rabbit anti-Smad7 antibody (HA610387) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: COS-1 cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: C2C12 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: NR8383 cell lysate (20 µg/Lane) Lane 9: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610387) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.