PDGFR beta Recombinant Antibody [SY10-08] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610390
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P |
| Clone number: | SY10-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Immunogen: | Recombinant protein within Human PDGFR beta aa 961-1,106 / 1,106. |
| Positive control: | SH-SY5Y cell lysate, C2C12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, human spleen tissue, mouse brain tissue, mouse spleen tissue, rat brain tissue, rat spleen tissue. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Lysosome lumen. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:10,000 1:500 1:500-1:2,000 |
| Uniprot #: | SwissProt: P09619 Human | P05622 Mouse | Q05030 Rat |
| Alternative names: | Beta platelet derived growth factor receptor Beta-type platelet-derived growth factor receptor CD 140B CD140 antigen-like family member B CD140b CD140b antigen IBGC4 IMF1 JTK12 OTTHUMP00000160528 PDGF R beta PDGF-R-beta PDGFR 1 PDGFR PDGFR beta PDGFR1 PDGFRB PGFRB_HUMAN Platelet derived growth factor receptor 1 Platelet derived growth factor receptor beta Platelet derived growth factor receptor beta polypeptide |
Images
|
Fig1:
Western blot analysis of PDGFR beta on different lysates with Mouse anti-PDGFR beta antibody (HA610390) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (10 µg/Lane) Lane 2: C2C12 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610390) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig3:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-PDGFR beta antibody (HA610390) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610390) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-PDGFR beta antibody (HA610390) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610390) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-PDGFR beta antibody (HA610390) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610390) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-PDGFR beta antibody (HA610390) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610390) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-PDGFR beta antibody (HA610390) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610390) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig9:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-PDGFR beta antibody (HA610390) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610390) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.