PDGFR beta Recombinant Antibody [SY10-08] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610391
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P |
| Clone number: | SY10-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Immunogen: | Recombinant protein within Human PDGFR beta aa 961-1,106 / 1,106. |
| Positive control: | SH-SY5Y cell lysate, C2C12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human spleen tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Lysosome lumen. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:10,000 1:200 1:200-1:1,000 |
| Uniprot #: | SwissProt: P09619 Human | P05622 Mouse | Q05030 Rat |
| Alternative names: | Beta platelet derived growth factor receptor Beta-type platelet-derived growth factor receptor CD 140B CD140 antigen-like family member B CD140b CD140b antigen IBGC4 IMF1 JTK12 OTTHUMP00000160528 PDGF R beta PDGF-R-beta PDGFR 1 PDGFR PDGFR beta PDGFR1 PDGFRB PGFRB_HUMAN Platelet derived growth factor receptor 1 Platelet derived growth factor receptor beta Platelet derived growth factor receptor beta polypeptide |
Images
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Fig1:
Western blot analysis of PDGFR beta on different lysates with Rat anti-PDGFR beta antibody (HA610391) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (10 µg/Lane) Lane 2: C2C12 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 1 minute 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610391) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rat anti-PDGFR beta antibody (HA610391) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610391) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-PDGFR beta antibody (HA610391) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610391) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rat anti-PDGFR beta antibody (HA610391) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610391) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.