NF-H Recombinant Antibody [JF99-07] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
cat.: HA610393
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P |
| Clone number: | JF99-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Immunogen: | Synthetic peptide within human NEFH aa 690-740. |
| Positive control: | Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, axon. |
| Recommended Dilutions:
IHC-P |
1:2,000 |
| Uniprot #: | SwissProt: P12036 Human | P19246 Mouse | P16884 Rat |
| Alternative names: | 200 kDa neurofilament protein CMT2CC Nefh Neurofilament heavy polypeptide 200kDa Neurofilament heavy polypeptide Neurofilament triplet H protein NF H NF-H NFH NFH_HUMAN |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Guinea pig anti-NF-H antibody (HA610393) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610393) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Guinea pig anti-NF-H antibody (HA610393) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610393) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Guinea pig anti-NF-H antibody (HA610393) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610393) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.