NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610394
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clone number: | JF99-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Immunogen: | Synthetic peptide within human NEFH aa 690-740. |
| Positive control: | Mouse cerebellum tissue lysate, Rat brain tissue lysate, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, axon. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:500-1:2,000 1:500 |
| Uniprot #: | SwissProt: P12036 Human | P19246 Mouse | P16884 Rat |
| Alternative names: | 200 kDa neurofilament protein CMT2CC Nefh Neurofilament heavy polypeptide 200kDa Neurofilament heavy polypeptide Neurofilament triplet H protein NF H NF-H NFH NFH_HUMAN |
Images
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Fig1:
Western blot analysis of NF-H on different lysates with Rat anti-NF-H antibody (HA610394) at 1/2,000 dilution. Lane 1: Mouse cerebellum tissue lysate (10 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 112 kDa Observed band size: 200 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610394) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-H antibody (HA610394) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610394) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NF-H antibody (HA610394) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610394) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Application: IF-Tissue Species: Human Site: brain Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.