PINK1 Recombinant Mouse Monoclonal Antibody [PSH22-50]
cat.: HA610397
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH22-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG1 |
| Positive control: | Mouse heart tissue lysate, Mouse liver tissue lysate, Rat placenta tissue lysate, Rat heart tissue lysate, HeLa treated with 10μM CCCP for 24 hours cell lysate. |
| Subcellular location: | SwissProt: Q9BXM7 Human SwissProt: Q99MQ3 Mouse SwissProt: B5DFG1 Rat |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9BXM7 Human | Q99MQ3 Mouse | B5DFG1 Rat |
| Alternative names: | BRPK FLJ27236 mitochondrial PARK 6 PARK6 Phosphatase and Tensin Homolog PINK 1 PINK1 PINK1_HUMAN Protein kinase BRPK PTEN induced putative kinase 1 PTEN induced putative kinase protein 1 PTEN-induced putative kinase protein 1 Serine/threonine kinase PINK1 mitochondrial Serine/threonine protein kinase PINK1 mitochondrial Serine/threonine-protein kinase PINK1 |
Images
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Fig1:
Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA610397) at 1/5,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat placenta tissue lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610397) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of PINK1 on different lysates with Mouse anti-PINK1 antibody (HA610397) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610397) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.