CD68 Recombinant Antibody [PSH06-65] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610399
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IF-Cell, IF-Tissue |
| Clone number: | PSH06-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Immunogen: | Recombinant protein within mouse CD68 aa 1-316. |
| Positive control: | RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Endosome membrane, Lysosome membrane; Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IF-Cell IF-Tissue |
1:2,000 1:200 1:500 1:500 1:200 |
| Uniprot #: | SwissProt: P31996 Mouse Entrez Gene: 287435 Rat |
| Alternative names: | CD 68 CD68 CD68 antigen CD68 molecule CD68_HUMAN DKFZp686M18236 gp11 Gp110 LAMP4 Macrophage antigen CD68 (microsialin) MACROPHAGE ANTIGEN CD68 Macrosialin SCARD1 Scavenger receptor class D member 1 |
Images
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Fig1:
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA610399) at 1/2,000 dilution. Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate (10 µg/Lane) Lane 2: RAW264.7 treated with PNGase F cell lysate (10 µg/Lane) Lane 3: Mouse spleen tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Exposure time: 20 seconds; ECL: K1801 CD68 is a glycoprotein and can be deglycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 7680921) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610399, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 35 kDa Observed band size: 60-100 kDa |
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Fig2:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Spleen Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA610399, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
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Fig3:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Spleen Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA610399, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610399, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610399, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610399, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610399, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
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Fig8:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: RAW264.7 (Mouse monocytic macrophage leukemia cells)/NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610399, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). Negative control: NIH/3T3 (PMID: 29631164). |
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Fig9:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610399, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
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Fig10:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610399, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.