LAMP1 Recombinant Antibody [PSH07-39] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610400
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clone number: | PSH07-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 0.5ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Immunogen: | Synthetic peptide within mouse LAMP1 aa 371-406. |
| Subcellular location: | Lysosome membrane, Endosome membrane, Late endosome membrane, Cell membrane, Cytolytic granule membrane. |
| Uniprot #: | SwissProt: P11279 Human | P11438 Mouse | P14562 Rat |
| Alternative names: | CD107 antigen like family member A CD107 antigen-like family member A CD107a CD107a antigen LAMP 1 LAMP-1 LAMP1 LAMP1_HUMAN LAMPA LGP120 lgpA Lysosomal membrane glycoprotein 120KD Lysosomal Associated Membrane Protein 1 Lysosome associated membrane glycoprotein 1 Lysosome-associated membrane glycoprotein 1 Lysosome-associated membrane protein 1 OTTHUMP00000040663 |
Images
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Fig1:
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA610400) at 1/5,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells) Lane 2: A431 treated with PNGaseF Lane 3: HeLa (Human cervical adenocarcinoma cells) Lane 4: NIH/3T3 (Mouse fibroblasts) Lysates/proteins at 10 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Expression pattern of LAMP1 protein in A431 is consistent with the literature (PMID: 28456975). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610400, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44 kDa Observed band size: 110/44 kDa |
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Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig7:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
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Fig8:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
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Fig9:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610400, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.