4-Hydroxynonenal Recombinant Mouse Monoclonal Antibody [PSH23-22]
cat.: HA610401
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH23-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Positive control: | HeLa treated with 0.2mg/mL 4-HNE for 30min cell lysate. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:500 |
| Alternative names: | (2E)-4-Hydroxy-2-nonenal 2-Nonenal, 4-hydroxy-, (2E,4R)- 4 HNE 4Hydroxynonenal 4-Hydroxynonenal 4-hydroxy-trans-2-nonenal HNE 4-HNE 4 Hydroxynonenal |
Images
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Fig1:
Western blot analysis of 4-Hydroxynonenal on different lysates with Mouse anti-4-Hydroxynonenal antibody (HA610401) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells）cell lysate Lane 2: HeLa treated with 0.2mg/mL 4-HNE for 30min cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610401, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 35-150 kDa Observed band size: 35-150 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 4-HNE(0.2mg/ml 30min) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610401, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.