Myelin Basic Protein Recombinant Antibody [JF0943] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610404
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | JF0943 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Immunogen: | Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Myelin membrane, Nucleus. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:5,000 1:5,000 |
| Uniprot #: | SwissProt: P04370 Mouse | P02688 Rat |
| Alternative names: | GDB Golli MBP Golli MBP; myelin basic protein Hemopoietic MBP HMBPR HUGO MBP MBP_CAVPO MBP_HUMAN MGC99675 MLD Myelin A1 protein Myelin A1 Protein, basic Myelin basic protein Myelin Deficient Myelin membrane encephalitogenic protein OTTHUMP00000163776 OTTHUMP00000174387 OTTHUMP00000174388 SHI Shiverer SP |
Images
|
Fig1:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Cerebral cortex Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBS Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA610404, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cerebral cortex Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610404, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610404, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
|
Fig4:
Western blot analysis of Myelin Basic Protein on different lysates with Rat anti-Myelin Basic Protein antibody (HA610404) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 8 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610404, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33 kDa Observed band size: 14-20 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.