PARK7 Recombinant Mouse Monoclonal Antibody [PSH23-76]
cat.: HA610411
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH23-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG1 |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, A549 (Human lung adenocarcinoma cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Nucleus, Membrane raft, Mitochondrion, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:500 1:5,000 1:2,000 |
| Uniprot #: | SwissProt: Q99497 Human |
| Alternative names: | CAP1 DJ-1 DJ1 DJ1 protein Epididymis secretory sperm binding protein Li 67p FLJ27376 FLJ34360 FLJ92274 HEL S 67p Oncogene DJ1 OTTHUMP00000001348 OTTHUMP00000001349 OTTHUMP00000001350 OTTHUMP00000001351 PARK7 PARK7_HUMAN Parkinson disease (autosomal recessive, early onset) 7 Parkinson disease protein 7 Parkinson protein 7 Protein DJ-1 SP22 |
Images
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Fig1:
Western blot analysis of PARK7 on different lysates with Mouse anti-PARK7 antibody (HA610411) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: A549 (Human lung adenocarcinoma cells) cell lysate Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610411, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20 kDa Observed band size: 20 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610411, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610411, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
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Fig4:
Application: Flow Cytometry (Intra) Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA610411 (1/2,000) (Red) compared with Mouse IgG1 Isotype Control (HA601336, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.