Cytokeratin 10 Recombinant Antibody [ST05-43] - Mouse IgG1 (Chimeric) - BSA and Azide free
cat.: HA610415
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, IF-Tissue |
| Clone number: | ST05-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Immunogen: | Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584. |
| Positive control: | Human Skin, Mouse Skin, Rat Skin. |
| Subcellular location: | Secreted, extracellular space, Cell surface, Cytoplasm. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P13645 Human | P02535 Mouse | Q6IFW6 Rat |
| Alternative names: | BCIE BIE CK 10 CK10 CK-10 Cytokeratin-10 EHK K10 K1C10_HUMAN Keratin 10 Keratin Keratin type i cytoskeletal 10 Keratin type I cytoskeletal 59 kDa Keratin-10 Keratin10 KPP KRT10 type I cytoskeletal 10 |
Images
|
Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610415, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610415, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610415, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1120, 20 minutes at room temperature. |
|
Fig4:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610415, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
|
Fig5:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610415, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
|
Fig6:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610415, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.