SST/Somatostatin 28 Recombinant Antibody [JE43-52] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610417
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-Fr, IHC-P |
| Clone number: | JE43-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Immunogen: | Synthetic peptide within Human Somatostatin 28 aa 67-116 / 116. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: P61278 Human | P60041 Mouse | P60042 Rat |
| Alternative names: | Growth hormone release inhibiting factor SMST Somatostatin 28 Somatostatin SST |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610417, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610417, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610417, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610417, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610417, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610417, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610417, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610417, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.