Ctip2 Recombinant Antibody [JF09-90] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610422
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P |
| Clone number: | JF09-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Immunogen: | Synthetic peptide within Human Ctip2 aa 491-534 / 894. |
| Positive control: | Jurkat (Human T-lymphoblastic cells) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, LO2 cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:5,000 1:2,000 1:10,000 |
| Uniprot #: | SwissProt: Q9C0K0 Human | Q99PV8 Mouse Entrez Gene: 314423 Rat |
| Alternative names: | ATL1 alpha ATL1 ATL1 beta ATL1 gamma ATL1-delta B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein B cell lymphoma/leukemia 11B B-cell CLL/lymphoma 11B (zinc finger protein) B-cell CLL/lymphoma 11B B-cell lymphoma/leukemia 11B BC11B_HUMAN BCL-11B Bcl11b BCL11B/TRDC fusion COUP TF interacting protein 2 COUP-TF interacting protein 2 COUP-TF-interacting protein 2 Ctip 2 CTIP-2 CTIP2 hRIT1 alpha hRit1 Radiation induced tumor suppressor gene 1 Radiation induced tumor suppressor gene 1 protein Radiation-induced tumor suppressor gene 1 protein Rit 1 Rit1 zinc finger protein hRit1 alpha ZNF856B |
Images
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Fig1:
Western blot analysis of Ctip2 on different lysates with Rat anti-Ctip2 antibody (HA610422) at 1/5,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 3: LO2 cell lysate Lane 4: Daudi (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610422, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 96 kDa Observed band size: 120 kDa |
|
Fig2:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: P0 brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA610422, 1/2,000, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610422, 1/10,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610422, 1/10,000, 1 hour at room temperature. Secondary antibody: HA1023, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.