TDP43 Recombinant Antibody [PSH09-14] - Rat IgG1 (Chimeric) - BSA and Azide free
cat.: HA610425
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IF-Cell |
| Clone number: | PSH09-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Immunogen: | Synthetic peptide within human TDP43 aa 365-414. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) lysate, HEK-293 (Human embryonic kidney cell) lysate, SH-SY5Y (Human neuroblastoma cell) lysate, K-562 (Human chronic myelogenous leukemia cell) lysate, C2C12 (Mouse myoblast) lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate, Mouse brain tissue lysate. |
| Subcellular location: | Cytoplasm, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IF-Cell |
1:2,000 1:500-1:2,000 1:500 1:50 |
| Uniprot #: | SwissProt: Q13148 Human | Q921F2 Mouse Entrez Gene: 298648 Rat |
| Alternative names: | ALS10 OTTHUMP00000002171 OTTHUMP00000002172 OTTHUMP00000002173 TADBP_HUMAN TAR DNA binding protein 43 TAR DNA binding protein TAR DNA-binding protein 43 TARDBP TDP 43 TDP-43 TDP43 |
Images
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Fig1:
Western blot analysis of TDP43 on different lysates with Rabbit anti-TDP43 antibody (HA610425) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) lysate Lane 2: HEK-293 (Human embryonic kidney cell) lysate Lane 3: SH-SY5Y (Human neuroblastoma cell) lysate Lane 4: K-562 (Human chronic myelogenous leukemia cell) lysate Lane 5: C2C12 (Mouse myoblast) lysate Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) lysate Lane 7: Mouse brain tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA610425, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rat IgG-HRP (HA1023), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 45 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610425, 1/500, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610425, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA610425, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1023, 30 minutes at room temperature. |
|
Fig5:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610425, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
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Fig6:
Application: Immunofluorescence (IF-tissue)
Species: Rat Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA610425, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature. |
|
Fig7:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610425, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
|
Fig8:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: C2C12 (Mouse myoblast) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA610425, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.